[Influence of adenovirus transfection on the maturation characteristics of human immature dendritic cells]

Zhonghua Shao Shang Za Zhi. 2006 Dec;22(6):458-61.
[Article in Chinese]

Abstract

Objective: To observe the changes in the phenotype characteristics and immune function after transfection of cord blood derived immature dendritic cells( imDC) with Adeasy-EGFP adenovirus vector, and to explore the function of IL-10 in inhibition of imDC maturation.

Methods: Immature dendritic cells were generated from human cord blood(CB) monocyte cultured with rhGM-CSF and rhIL-4. The recombinant adenovirus vector AdEASY-EGFP was transduced into immature dendritic cells on the third day with or without adding IL-10. The expression of cell maturation marker CD83, CD86 and HLA-DR were determined with flow cytometry. Allogeneic mixed leukocyte reaction( MLR) was used to examine the imDC's ability to promote T cell proliferation.

Results: The expression of surface maturation markers of imDC after transfection with adenovirus were significantly up-regulated ( CD86:46+/-10; CD83: 38 +/- 7; HLA-DR: 82 + 12) , and its ability to promote T cell proliferation was also obviously increased( SI > 2. 0). However, the expression of surface maturation markers of imDC after IL-10 treatment had lower mature phenotypes expression after transduction (CD86:8 +/- 5; CD83: 9 +/- 3; HLA-DR:63 +/- 12), and T cell stimulating ability was decreased comparing with adenovirus transfection groups.

Conclusion: Adenovirus can be transduced into imDC with high efficiency, but transfection itself can promote imDC's maturation. IL-10 treatment can inhibit the tendency to maturation stimulated by adenovirus transduction efficiently.

Publication types

  • English Abstract

MeSH terms

  • Adenoviridae / genetics
  • Cell Differentiation
  • Cells, Cultured
  • Dendritic Cells / cytology*
  • Genetic Vectors*
  • Humans
  • Interleukin-10 / pharmacology
  • T-Lymphocytes / metabolism
  • Transfection*

Substances

  • Interleukin-10