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1: Genome Biol. 2007;8(4):R55.Click here to read Click here to read Links

A major zebrafish polymorphism resource for genetic mapping.

Bradley KM, Elmore JB, Breyer JP, Yaspan BL, Jessen JR, Knapik EW, Smith JR.

Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232-0275, USA. Kevin.bradley@vanderbilt.edu

We have identified 645,088 candidate polymorphisms in zebrafish and observe a single nucleotide polymorphism (SNP) validation rate of 71% to 86%, improving with polymorphism confidence score. Variant sites are non-random, with an excess of specific novel T- and A-rich motifs. We positioned half of the polymorphisms on zebrafish genetic and physical maps as a resource for positional cloning. We further demonstrate bulked segregant analysis using the anchored SNPs as a method for high-throughput genetic mapping in zebrafish.

PMID: 17428331 [PubMed - indexed for MEDLINE]

PMCID: PMC1896001