Semin Cell Biol. 1991 Apr;2(2):117-29.

Bio Vision: microscopy in three dimensions.

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Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.

Abstract

Conventional electron microscopy is inadequate for visualizing the three-dimensional networks supporting cell architecture: the cytoskeleton and nuclear matrix. Consequently, we have not appreciated the extent to which the cell, its biochemistry, and its molecular biology are structured. A new technology combining in situ cell fractionation and resinless section electron microscopy allows the visualization of cell structure in three dimensions and permits the localization of individual components. These techniques reveal a far richer cell architecture than had been assumed and will allow important problems of biology, which have not surrendered their secrets to a purely biochemical approach, to be addressed.

PMID:: 1742476; [PubMed - indexed for MEDLINE]

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Immunolocalization in three dimensions: immunogold staining of cytoskeletal and nuclear matrix proteins in resinless electron microscopy sections. [Proc Natl Acad Sci U S A. 1990]
Immunolocalization in three dimensions: immunogold staining of cytoskeletal and nuclear matrix proteins in resinless electron microscopy sections.
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Cited by 2 PubMed Central articles

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Bio Vision: microscopy in three dimensions.

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