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Nat Methods. 2007 May;4(5):409-12. Epub 2007 Apr 8.

A genomic integration method to visualize localization of endogenous mRNAs in living yeast.

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  • 1Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel.


mRNA localization may be an important determinant for protein localization. We describe a simple PCR-based genomic-tagging strategy (m-TAG) that uses homologous recombination to insert binding sites for the RNA-binding MS2 coat protein (MS2-CP) between the coding region and 3' untranslated region (UTR) of any yeast gene. Upon coexpression of MS2-CP fused with GFP, we demonstrate the localization of endogenous mRNAs (ASH1, SRO7, PEX3 and OXA1) in living yeast (Saccharomyces cerevisiae).

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