Warning: The NCBI web site requires JavaScript to function. more...

My NCBI Sign In

Science. 2007 Apr 6;316(5821):120-3.

An ATP gate controls tubulin binding by the tethered head of kinesin-1.

Alonso MC, Drummond DR, Kain S, Hoeng J, Amos L, Cross RA.

Source

Molecular Motors Group, Marie Curie Research Institute, The Chart, Oxted, Surrey RH8 0TL, UK.

Abstract

Kinesin-1 is a two-headed molecular motor that walks along microtubules, with each step gated by adenosine triphosphate (ATP) binding. Existing models for the gating mechanism propose a role for the microtubule lattice. We show that unpolymerized tubulin binds to kinesin-1, causing tubulin-activated release of adenosine diphosphate (ADP). With no added nucleotide, each kinesin-1 dimer binds one tubulin heterodimer. In adenylyl-imidodiphosphate (AMP-PNP), a nonhydrolyzable ATP analog, each kinesin-1 dimer binds two tubulin heterodimers. The data reveal an ATP gate that operates independently of the microtubule lattice, by ATP-dependent release of a steric or allosteric block on the tubulin binding site of the tethered kinesin-ADP head.

Comment in

Science. 2007 Apr 6;316(5821):58-9.

PMID:: 17412962; [PubMed - indexed for MEDLINE]
PMCID: PMC2504013

Free PMC Article

Images from this publication.See all images (5) Free text

Fig.5

Fig. 4

Fig. 3

Fig. 1

Fig. 2

LinkOut - more resources

Full Text Sources

Other Literature Sources

Evaluations and comments from leading biologists - Faculty of 1000 Biology

Supplemental Content

Click here to read

Click here to read

Related citations

A mechanism for microtubule depolymerization by KinI kinesins. [Mol Cell. 2002]
A mechanism for microtubule depolymerization by KinI kinesins.
Moores CA, Yu M, Guo J, Beraud C, Sakowicz R, Milligan RA. Mol Cell. 2002 Apr; 9(4):903-9.
A new look at the microtubule binding patterns of dimeric kinesins. [J Mol Biol. 2000]
A new look at the microtubule binding patterns of dimeric kinesins.
Hoenger A, Thormählen M, Diaz-Avalos R, Doerhoefer M, Goldie KN, Müller J, Mandelkow E. J Mol Biol. 2000 Apr 14; 297(5):1087-103.
Nucleotide-dependent structural changes in dimeric NCD molecules complexed to microtubules. [J Mol Biol. 1998]
Nucleotide-dependent structural changes in dimeric NCD molecules complexed to microtubules.
Hirose K, Cross RA, Amos LA. J Mol Biol. 1998 May 1; 278(2):389-400.
Review Kinesin: a molecular motor with a spring in its step. [Proc Biol Sci. 2002]
Review Kinesin: a molecular motor with a spring in its step.
Thomas N, Imafuku Y, Kamiya T, Tawada K. Proc Biol Sci. 2002 Nov 22; 269(1507):2363-71.
Review Kinesins and microtubules: their structures and motor mechanisms. [Curr Opin Cell Biol. 2000]
Review Kinesins and microtubules: their structures and motor mechanisms.
Sablin EP. Curr Opin Cell Biol. 2000 Feb; 12(1):35-41.

See reviews... See all...

Cited by 19 PubMed Central articles

Electrostatically biased binding of kinesin to microtubules. [PLoS Biol. 2011]
Electrostatically biased binding of kinesin to microtubules.
Grant BJ, Gheorghe DM, Zheng W, Alonso M, Huber G, Dlugosz M, McCammon JA, Cross RA. PLoS Biol. 2011 Nov; 9(11):e1001207. Epub 2011 Nov 29.
The kinesin-13 MCAK has an unconventional ATPase cycle adapted for microtubule depolymerization. [EMBO J. 2011]
The kinesin-13 MCAK has an unconventional ATPase cycle adapted for microtubule depolymerization.
Friel CT, Howard J. EMBO J. 2011 Aug 26; 30(19):3928-39. Epub 2011 Aug 26.
Review Mechanism of processive movement of monomeric and dimeric kinesin molecules. [Int J Biol Sci. 2010]
Review Mechanism of processive movement of monomeric and dimeric kinesin molecules.
Xie P. Int J Biol Sci. 2010 Nov 3; 6(7):665-74. Epub 2010 Nov 3.

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
References for this PMC Article
Citation referenced in PubMed article. Only valid for PubMed citations that are also in PMC.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Free in PMC
Free full text articles in PMC
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

An ATP gate controls tubulin binding by the tethered head of kinesin-1.
An ATP gate controls tubulin binding by the tethered head of kinesin-1.
Science. 2007 Apr 6 ;316(5821):120-3.

PubMed
Long-standing Morel-Lavallée lesions of the trochanteric region and proximal thi...
Long-standing Morel-Lavallée lesions of the trochanteric region and proximal thigh: MRI features in five patients.
AJR Am J Roentgenol. 2004 May ;182(5):1289-94.

PubMed
[Liver fructokinase].
[Liver fructokinase].
Biochim Biophys Acta. 1952 Apr ;8(4):416-23.

PubMed
Safety criteria for transcranial direct current stimulation (tDCS) in humans.
Safety criteria for transcranial direct current stimulation (tDCS) in humans.
Clin Neurophysiol. 2003 Nov ;114(11):2220-2; author reply 2222-3.

PubMed
The effects of precompetition massage on the kinematic parameters of 20-m sprint...
The effects of precompetition massage on the kinematic parameters of 20-m sprint performance.
J Strength Cond Res. 2010 May ;24(5):1179-83.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...

You are here: NCBI > Literature > PubMed

Write to the Help Desk