Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Apr 1;63(Pt 4):320-3. Epub 2007 Mar 30.

Expression, purification and preliminary X-ray crystallographic studies of the human immunodeficiency virus 1 subtype C protease.

Coman RM, Robbins A, Goodenow MM, McKenna R, Dunn BM.

Source

Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, FL 32610, USA.

Abstract

Crystals of the human immunodeficiency virus 1 (HIV-1) subtype C protease (PR) complexed with the clinically used inhibitors indinavir (IDV) and nelfinavir (NFV) have been grown in the monoclinic space group P2(1), with mean unit-cell parameters a = 46.7 (+/-0.1), b = 59.8 (+/-0.3), c = 87.0 (+/-0.4) A, beta = 95.2 (+/-0.5) degrees. The crystals of both complexes have been shown to diffract X-rays to 2.3 A resolution. The diffraction data for the subtype C PR complexes with IDV and NFV were subsequently processed and reduced, with overall R(sym) values of 8.4 and 11.4%, respectively. Based on the unit-cell volumes, molecular-replacement results and packing considerations, there are two protease homodimers per crystallographic asymmetric unit in each of the complexes. The data were initially phased using a model based on the crystal structure of HIV-1 subtype B PR; the structures have been determined and further refinement and analysis are in progress. These structures and subsequent studies with other inhibitors will greatly aid in correlating the amino-acid variation between the different HIV PRs and understanding their differential sensitivity and resistance to current drug therapy.

PMID:: 17401206; [PubMed - indexed for MEDLINE]
PMCID:: PMC2330223

Free PMC Article

Images from this publication.See all images (2) Free text

Publication Types, MeSH Terms, Substances, Grant Support

Publication Types

MeSH Terms

Substances

Grant Support

R37 AI028571/AI/NIAID NIH HHS/United States

LinkOut - more resources

Full Text Sources

Other Literature Sources

COS Scholar Universe

Supplemental Content

Icon for International Union of Crystallography

Save items

Related citations in PubMed

Production, purification and preliminary X-ray crystallographic studies of adeno-associated virus serotype 1. [Acta Crystallogr Sect F Struct...]
Production, purification and preliminary X-ray crystallographic studies of adeno-associated virus serotype 1.
Miller EB, Gurda-Whitaker B, Govindasamy L, McKenna R, Zolotukhin S, Muzyczka N, Agbandje-McKenna M. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Dec 1; 62(Pt 12):1271-4. Epub 2006 Nov 30.
High-resolution structure of unbound human immunodeficiency virus 1 subtype C protease: implications of flap dynamics and drug resistance. [Acta Crystallogr D Biol Crysta...]
High-resolution structure of unbound human immunodeficiency virus 1 subtype C protease: implications of flap dynamics and drug resistance.
Coman RM, Robbins AH, Goodenow MM, Dunn BM, McKenna R. Acta Crystallogr D Biol Crystallogr. 2008 Jul; D64(Pt 7):754-63. Epub 2008 Jun 18.
Crystallographic analysis of human immunodeficiency virus 1 protease with an analog of the conserved CA-p2 substrate -- interactions with frequently occurring glutamic acid residue at P2' position of substrates. [Eur J Biochem. 1997]
Crystallographic analysis of human immunodeficiency virus 1 protease with an analog of the conserved CA-p2 substrate -- interactions with frequently occurring glutamic acid residue at P2' position of substrates.
Weber IT, Wu J, Adomat J, Harrison RW, Kimmel AR, Wondrak EM, Louis JM. Eur J Biochem. 1997 Oct 15; 249(2):523-30.
Review Preliminary crystallographic studies of the ligand-binding domain of the thyroid hormone receptor complexed with triiodothyronine. [J Mol Biol. 1994]
Review Preliminary crystallographic studies of the ligand-binding domain of the thyroid hormone receptor complexed with triiodothyronine.
McGrath ME, Wagner RL, Apriletti JW, West BL, Ramalingam V, Baxter JD, Fletterick RJ. J Mol Biol. 1994 Mar 25; 237(2):236-9.
Review Polymorphisms in HIV-1 subtype C proteases and the potential impact on protease inhibitors. [Trop Med Int Health. 2008]
Review Polymorphisms in HIV-1 subtype C proteases and the potential impact on protease inhibitors.
Bessong PO. Trop Med Int Health. 2008 Feb; 13(2):144-51.

See reviews... See all...

Cited by 1 PubMed Central article

Structure of the unbound form of HIV-1 subtype A protease: comparison with unbound forms of proteases from other HIV subtypes. [Acta Crystallogr D Biol Crysta...]
Structure of the unbound form of HIV-1 subtype A protease: comparison with unbound forms of proteases from other HIV subtypes.
Robbins AH, Coman RM, Bracho-Sanchez E, Fernandez MA, Gilliland CT, Li M, Agbandje-McKenna M, Wlodawer A, Dunn BM, McKenna R. Acta Crystallogr D Biol Crystallogr. 2010 Mar; 66(Pt 3):233-42. Epub 2010 Feb 12.

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
References for this PMC Article
Citation referenced in PubMed article. Only valid for PubMed citations that are also in PMC.
Free in PMC
Free full text articles in PMC
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Expression, purification and preliminary X-ray crystallographic studies of the h...
Expression, purification and preliminary X-ray crystallographic studies of the human immunodeficiency virus 1 subtype C protease.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Apr 1 ;63(Pt 4):320-3. Epub 2007 Mar 30 .

PubMed
Effects of prolonged treatment with the opiate tramadol on prodynorphin gene exp...
Effects of prolonged treatment with the opiate tramadol on prodynorphin gene expression in rat CNS.
J Mol Neurosci. 2006 ;30(3):341-7.

PubMed
Neuroprotection from secondary injury by polyethylene glycol requires its intern...
Neuroprotection from secondary injury by polyethylene glycol requires its internalization.
J Exp Biol. 2007 Apr ;210(Pt 8):1455-62.

PubMed
Physical and mental comorbidity, disability, and suicidal behavior associated wi...
Physical and mental comorbidity, disability, and suicidal behavior associated with posttraumatic stress disorder in a large community sample.
Psychosom Med. 2007 Apr ;69(3):242-8. Epub 2007 Mar 30 .

PubMed
A missense mutation in the vasopressin-neurophysin precursor gene cosegregates w...
A missense mutation in the vasopressin-neurophysin precursor gene cosegregates with human autosomal dominant neurohypophyseal diabetes insipidus.
EMBO J. 1992 Jan ;11(1):19-23.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: