Your browser version may not work well with NCBI's Web applications. More information here...
1: Genome Biol. 2007 Mar 26;8(3):R42. [Epub ahead of print]Click here to read Click here to read Links

CAGE-TSSchip: promoter-based expression profiling using the 5'-leading label of capped transcripts.

Katayama S, Kanamori-Katayama M, Yamaguchi K, Carninci P, Hayashizaki Y.

Laboratory for Genome Exploration Research Group, Genomic Sciences Center, RIKEN Yokohama Institute, Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan. rgscerg@gsc.riken.jp.

ABSTRACT: Cap analysis gene expression (CAGE) technology has revealed numerous transcription start sites (TSSs) in mammals and has suggested complex promoter-based patterns of regulation. We developed the CAGE-TSSchip to detect promoter-based transcriptional activity. The CAGE-TSSchip is a customized oligonucleotide array that targets known TSSs identified by CAGE. A new labeling method, labeling capped transcripts from the 5'-end, had to be developed. The CAGE-TSSchip is accurate and sensitive, and represents the activity of each TSS.

PMID: 17381848 [PubMed - as supplied by publisher]

PMCID: PMC1868931