Gingival crevicular fluid interleukin-1beta, prostaglandin E2 and periodontal status in a community population

J Clin Periodontol. 2007 Apr;34(4):285-93. doi: 10.1111/j.1600-051X.2007.01057.x.

Abstract

Aim: Interleukin-1 beta (IL-1beta) and prostaglandin E(2) (PGE(2)) are key inflammatory mediators involved in periodontal disease. The purposes of this molecular cross-sectional epidemiological study were to investigate relationships in a community sample between mean concentrations of IL-1beta and PGE(2) in gingival crevicular fluid (GCF) and (1) clinical periodontal signs and (2) risk factors of host inflammatory response and/or periodontal disease.

Material and methods: The sample comprised 6277 community-dwelling adults aged 52-74 years enrolled in the Atherosclerosis Risk in Communities (ARIC) study. IL-1beta and PGE(2) concentrations were measured using enzyme-linked immunosorbent assay. Person-level summary variables were computed for maximum pocket depth (MaxPD), maximum clinical attachment level (MaxCAL) and presence/absence of bleeding on probing (BOP). Mean GCF IL-1beta and PGE(2) concentrations were dependent variables in multiple linear regression models with periodontal measures and covariates as explanatory variables.

Results: Both GCF IL-1beta and PGE(2) were positively related to MaxPD and BOP in multiple regression models (p<0.01). Increased levels of IL-1beta and PGE(2) were associated with body mass index >or=30 kg/m(2).

Conclusion: Higher levels of GCF IL-1beta and PGE(2) were significantly associated with clinical signs of periodontal disease and independently related to patient-based anthropomorphic measures, behaviours and exposures in community-dwelling adults.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Aged
  • Body Mass Index
  • Cross-Sectional Studies
  • Dinoprostone / analysis
  • Dinoprostone / metabolism*
  • Female
  • Gingival Crevicular Fluid / chemistry*
  • Humans
  • Interleukin-1beta / analysis
  • Interleukin-1beta / metabolism*
  • Least-Squares Analysis
  • Linear Models
  • Male
  • Maryland / epidemiology
  • Middle Aged
  • Minnesota / epidemiology
  • Mississippi / epidemiology
  • Molecular Epidemiology
  • North Carolina / epidemiology
  • Periodontal Diseases / epidemiology*
  • Periodontal Diseases / metabolism*
  • Periodontal Index
  • Smoking / metabolism

Substances

  • Interleukin-1beta
  • Dinoprostone