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Mol Cell Biol. 2007 May;27(10):3612-24. Epub 2007 Mar 12.

Efficient RNA polyuridylation by noncanonical poly(A) polymerases.

Author information

  • 1Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom.

Abstract

Nuclear poly(A) polymerase (PAP) polyadenylates nascent mRNAs, promoting their nuclear export, stability, and translation, while the related cytoplasmic polymerase GLD-2 activates translation of deadenylated mRNAs. Here we characterize the biochemical activity of fission yeast Schizosaccharomyces pombe Cid1, a putative cytoplasmic PAP implicated in cell cycle checkpoint controls. Surprisingly, Cid1 has robust poly(U) polymerase activity in vitro, especially when isolated in native multiprotein complexes. Furthermore, we found that upon S-phase arrest, the 3' ends of actin mRNAs were posttranscriptionally uridylated in a Cid1-dependent manner. Finally, Hs2 (ZCCHC6), a human ortholog of Cid1, shows similar activity. These data suggest that uridylation of mRNA forms the basis of an evolutionarily conserved mechanism of gene regulation.

PMID:
17353264
[PubMed - indexed for MEDLINE]
PMCID:
PMC1899984
Free PMC Article

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