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PCR amplification of specific alleles (PASA) is a general method for rapidly detecting known single-base changes.
Dept. of Biochemistry and Molecular Biology, Mayo Clinic/Foundation, Rochester, MN 55905.
Previous data suggested that PCR amplification of specific alleles (also known as allele-specific amplification and amplification refractory mutation system) is not a general method for rapidly and accurately detecting known single-base changes. Herein we present our experience with the use of PCR amplification of specific alleles to detect 69 polymorphic or mutant alleles. Our results indicate that with proper optimization, all alleles were reliably distinguished.
PMID: 1734929 [PubMed - indexed for MEDLINE]
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Cited by 18 PubMed Central articles
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Evidence for X-chromosomal schizophrenia associated with microRNA alterations.
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[PLoS One. 2009]
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Wang JR, Wei YM, Long XY, Yan ZH, Nevo E, Baum BR, Zheng YL.
BMC Evol Biol. 2008 Mar 24; 8:91. Epub 2008 Mar 24.
[BMC Evol Biol. 2008]
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Haplotype and functional analysis of four flavin-containing monooxygenase isoform 2 (FMO2) polymorphisms in Hispanics.
Krueger SK, Siddens LK, Henderson MC, Andreasen EA, Tanguay RL, Pereira CB, Cabacungan ET, Hines RN, Ardlie KG, Williams DE.
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[Pharmacogenet Genomics. 2005]
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