Nature. 1992 Jan 30;355(6359):464-7.

A cDNA encoding RAP74, a general initiation factor for transcription by RNA polymerase II.

Finkelstein A, Kostrub CF, Li J, Chavez DP, Wang BQ, Fang SM, Greenblatt J, Burton ZF.

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Department of Biochemistry, Michigan State University, E. Lansing 48824.

Abstract

RAP30/74 (also known as TFIIF, beta gamma and FC is one of several general factors required for initiation by RNA polymerase II. The small RAP30 subunit of RAP30/74 binds directly to polymerase and appears structurally and functionally homologous to bacterial sigma factors in their RNA polymerase-binding region. RAP30/74 or recombinant RAP30 suppresses nonspecific binding of RNA polymerase II to DNA and is required for RNA polymerase II to assemble stably into a preinitiation complex containing promoter DNA and the general factors TFIID, TFIIA and TFIIB; both RAP30 and RAP74 are physical components of the preinitiation complex. A complementary DNA encoding human RAP30 has been isolated, and here we report the isolation of a cDNA encoding human RAP74. RAP30 and RAP74 produced in Escherichia coli can be used in place of natural human RAP30/74 to direct accurate transcription initiation by RNA polymerase II in vitro.

PMID:: 1734284; [PubMed - indexed for MEDLINE]

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The general transcription factor RAP30 binds to RNA polymerase II and prevents it from binding nonspecifically to DNA. [Mol Cell Biol. 1992]
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A cDNA encoding RAP74, a general initiation factor for transcription by RNA poly...
A cDNA encoding RAP74, a general initiation factor for transcription by RNA polymerase II.
Nature. 1992 Jan 30 ;355(6359):464-7.

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