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    Appl Environ Microbiol. 2007 May;73(9):3105-8. Epub 2007 Mar 2.

    Bacterial degradation of N,N-diethyl-m-toluamide (DEET): cloning and heterologous expression of DEET hydrolase.

    Rivera-Cancel G, Bocioaga D, Hay AG.

    Source

    Department of Microbiology, Cornell University, Ithaca, NY 14853, USA.

    Abstract

    Pseudomonas putida DTB grew aerobically with N,N-diethyl-m-toluamide (DEET) as a sole carbon source, initially breaking it down into 3-methylbenzoate and diethylamine. The former was further metabolized via 3-methylcatechol and meta ring cleavage. A gene from DTB, dthA, was heterologously expressed and shown to encode the ability to hydrolyze DEET into 3-methylbenzoate and diethylamine.

    PMID:
    17337538
    [PubMed - indexed for MEDLINE]
    PMCID: PMC1892861
    Free PMC Article

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