On-line liquid chromatography-electrospray ionisation mass spectrometry for kappa-carrageenan oligosaccharides with a porous graphitic carbon column

Aristotelis Antonopoulos; Patrick Favetta; William Helbert; Michel Lafosse

doi:10.1016/j.chroma.2007.02.023

On-line liquid chromatography-electrospray ionisation mass spectrometry for kappa-carrageenan oligosaccharides with a porous graphitic carbon column

J Chromatogr A. 2007 Apr 13;1147(1):37-41. doi: 10.1016/j.chroma.2007.02.023. Epub 2007 Feb 11.

Authors

Aristotelis Antonopoulos¹, Patrick Favetta, William Helbert, Michel Lafosse

Affiliation

¹ Institut de Chimie Organique et Analytique (ICOA), UMR CNRS 6005, Université d'Orléans, B.P. 6759, 45067 Orléans Cedex 2, France.

PMID: 17336315
DOI: 10.1016/j.chroma.2007.02.023

Abstract

Enzymatically digested oligosaccharides of kappa-carrageenans were separated on a porous graphitic carbon (PGC) column and characterised on-line by electrospray ionisation mass spectrometry (ESI-MS). Two different developing ions were applied. Among them ammonium hydrogencarbonate showed more eluting power as it should on normal anion-exchange stationary phases. The oligosaccharides were detected by ESI-MS as fully deprotonated oligosaccharides.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anion Exchange Resins
Carbon / chemistry*
Carrageenan / analysis*
Carrageenan / chemistry
Chromatography, High Pressure Liquid / instrumentation
Chromatography, High Pressure Liquid / methods
Graphite / chemistry*
Oligosaccharides / analysis*
Oligosaccharides / chemistry
Porosity
Spectrometry, Mass, Electrospray Ionization / methods*

Substances

Anion Exchange Resins
Oligosaccharides
Carbon
Graphite
Carrageenan