Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Science. 2007 Mar 2;315(5816):1270-4.

Reconstitution of DNA segregation driven by assembly of a prokaryotic actin homolog.

Author information

  • 1Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94158, USA.

Abstract

Multiple unrelated polymer systems have evolved to partition DNA molecules between daughter cells at division. To better understand polymer-driven DNA segregation, we reconstituted the three-component segregation system of the R1 plasmid from purified components. We found that the ParR/parC complex can construct a simple bipolar spindle by binding the ends of ParM filaments, inhibiting dynamic instability, and acting as a ratchet permitting incorporation of new monomers and riding on the elongating filament ends. Under steady-state conditions, the dynamic instability of unattached ParM filaments provides the energy required to drive DNA segregation.

PMID:
17332412
[PubMed - indexed for MEDLINE]
PMCID:
PMC2851738
Free PMC Article

Images from this publication.See all images (4)Free text

Fig. 1
Fig. 2
Fig. 3
Fig. 4
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Write to the Help Desk