Jpn J Infect Dis. 2007 Feb;60(1):45-7.

Construction of a pair of practical Nocardia-Escherichia coli shuttle vectors.

Chiba K, Hoshino Y, Ishino K, Kogure T, Mikami Y, Uehara Y, Ishikawa J.

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Department of Bioactive Molecules, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.

Abstract

We constructed a pair of Nocardia-Escherichia coli shuttle vectors, pNV18 and pNV19, by combining the mycobacterial plasmid pAL5000 with the E. coli vector pK18 or pK19. These vectors have a number of useful features, including small size (4.4 kb), a multiple cloning site, and blue/white selection. To our knowledge, pNV18 and pNV19 are the first cloning vectors for practical use in Nocardia spp.

PMID:: 17314425; [PubMed - indexed for MEDLINE]

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