A Cartesian plot depicting radiation hybrid typing generated by the Illumina® BeadStation. (a) Clones located under the sweeping arc from 0.30 on the y-axis to 0.30 on the x-axis were identified as negatives and labelled no calls (U). Once selected, the negative cluster was excluded and the calling algorithm was rerun. Clones containing BB genotypes were scored as present. Clones containing U genotypes were scored as absent. (b) Clones located under the sweeping arc from 0.40 on the y-axis to 0.40 on the x-axis were identified as negatives and labelled no calls (U). Once selected, the negative cluster was excluded and the calling algorithm was rerun. Clones containing A, H and B genotypes were scored as present. Clones containing U genotypes were scored as absent. (c) Clones located under the sweeping arc from 0.10 on the y-axis to 0.10 on the x-axis were identified as negatives and labelled no calls (U). Once selected, the negative cluster was excluded and the calling algorithm was rerun. One clone was labelled as ambiguous and marked as ‘2’. It is located at approximately 0 on the y-axis and just to the right of 0.2 on the x-axis. Clones containing A, H and B genotypes were scored as present. Clones containing U genotypes were scored as absent. Clones marked as ‘2’ were scored as ambiguous.