Nat Methods. 2007 Mar;4(3):215-7. Epub 2007 Feb 4.

Focused-ion-beam thinning of frozen-hydrated biological specimens for cryo-electron microscopy.

Marko M, Hsieh C, Schalek R, Frank J, Mannella C.

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Resource for Visualization of Biological Complexity, Wadsworth Center, Empire State Plaza, Albany, New York 12201, USA. marko@wadsworth.org

Abstract

Cryo-electron microscopy can provide high-resolution structural information about cells and organelles in the nearly native, frozen-hydrated state. Applicability, however, is limited by difficulties encountered in preparing suitably thin, vitreously frozen biological specimens. We demonstrate, by cryo-electron tomography of Escherichia coli cells, that a focused ion beam (FIB) can be used to thin whole frozen-hydrated cells in a convenient and essentially artifact-free way.

PMID:: 17277781; [PubMed - indexed for MEDLINE]

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