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Cytogenet Genome Res. 2007;116(1-2):110-2.

Localization of the single copy gene Mdh2 on Xenopus tropicalis chromosomes by FISH-TSA.

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  • 1Charles University in Prague, Faculty of Science, Department of Physiology and Developmental Biology, Prague, Czech Republic. vkrylov@natur.cuni.cz

Abstract

A single copy gene, mitochondrial malate dehydrogenase 2 (Mdh2), was localized on Xenopus tropicalis chromosomes by fluorescence in situ hybridization coupled with tyramide signal amplification (FISH-TSA). The respective cDNA was cloned and sequenced. The labeled probe hybridized with a subcentromeric region of the long arms of homologous chromosomes 3. Results of comparison of the gene localization with previously mapped X. laevis paralogs strongly suggest a common evolutionary origin of chromosomes 3 and 8 in X. laevis and chromosome 3 in X. tropicalis. This is the first time that a single copy gene has been visualized on X. tropicalis chromosomes. The FISH-TSA method gives a strong signal with a 1-kb labeled probe.

Copyright 2007 S. Karger AG, Basel.

PMID:
17268187
[PubMed - indexed for MEDLINE]
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