Roum Arch Microbiol Immunol. 1991 Jul-Sep;50(3):187-92.

Anti-phosphotyrosine antibodies--obtaining and purification.

Stefănescu M, Cremer L, Cristescu C, Matache C, Szegli G.

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Cantacuzino Institute, Bucharest, Romania.

Abstract

Anti-phosphotyrosine antibodies were obtained by rabbits immunization using bovine serum albumin-phosphotyrosine complex. Hyperimmune rabbit serum was purified on affinity chromatography column obtained by phosphotyramine binding on Sepharose 4 B, CNBr-activated. The antibodies obtained after purification were tested by ELISA and immunoblotting.

PMID:: 1726504; [PubMed - indexed for MEDLINE]

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L-TYROSINE - HSDB

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Anti-phosphotyrosine antibodies--obtaining and purification.

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