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Appl Environ Microbiol. 2007 Mar;73(6):1803-8. Epub 2007 Jan 19.

Simple method for repurification of endotoxins for biological use.

Author information

  • 1Equipe "Endotoxines," UMR 8619 du CNRS, IBBMC, Université de Paris-Sud, 91405 Orsay, France. Martine.Caroff@ibbmc.u-psud.fr

Abstract

A method for obtaining highly purified endotoxin (lipopolysaccharide [LPS]) in a few hours by repurification of commercial or laboratory preparations was devised. It avoids the use of phenol, which is not suitable for phenol-soluble lipopolysaccharides nor for some industrial purposes. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and matrix-assisted laser desorption ionization mass spectrometry analysis confirmed the integrity of the purified LPSs. The purified products did not activate Toll-like receptor 2 (TLR2), nuclear oligomerization domain 1 (NOD1), or NOD2 but did activate TLR4. Applied to different lipopolysaccharides, the method also improved their mass spectra, thus facilitating their structural analysis.

PMID:
17261511
[PubMed - indexed for MEDLINE]
PMCID:
PMC1828799
Free PMC Article

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