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Phytochem Anal. 2007 Jan-Feb;18(1):42-9.

Quantification of spiroether isomers and herniarin of different parts of Matricaria matricarioides and flowers of Chamaemelum nobile.

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  • 1School of Pharmacy, Memorial University of Newfoundland, St. John's, Newfoundland and Labrador, Canada A1B 3V6.

Abstract

A simple HPLC-PAD-MS method was established to quantitatively analyse two spiroether isomers (cis-en-yn-dicycloether and trans-en-yn-dicycloether) and the main coumarin, herniarin, in chamomile herbs, simultaneously. By using this method, the contents of these three compounds in the flowers of two chamomile species, Roman chamomile (Chamaemelum nobile) and pineapple weed (Matricaria matricarioides), as well as in different parts of pineapple weed, were investigated. It was found that the flowers of both herbs contained large amounts of cis-en-yn-dicycloether and trans-en-yn-dicycloether, with the trans-form being more abundant than the cis-form. The leaves of pineapple weed were found to have the highest concentration of cis-en-yn-dicycloether and herniarin than the other parts. HPLC-PAD-MS-guided isolation and identification of other constituents are also discussed.

PMID:
17260697
[PubMed - indexed for MEDLINE]
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