Format

Send to:

Choose Destination
See comment in PubMed Commons below
Nat Methods. 2007 Feb;4(2):127-9. Epub 2007 Jan 28.

Improved calcium imaging in transgenic mice expressing a troponin C-based biosensor.

Author information

  • 1Max Planck Institute of Neurobiology, Am Klopferspitz 18, 82152 Martinsried, Germany.

Abstract

Fluorescent Ca(2+) indicator proteins (FCIPs) are attractive tools for studying Ca(2+) dynamics in live cells. Here we describe transgenic mouse lines expressing a troponin C (TnC)-based biosensor. The biosensor is widely expressed in neurons and has improved Ca(2+) sensitivity both in vitro and in vivo. This allows FCIP-based two-photon Ca(2+) imaging of distinct neurons and their dendrites in vivo, and opens a new avenue for structure-function analysis of intact neuronal circuits.

PMID:
17259991
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Nature Publishing Group
    Loading ...
    Write to the Help Desk