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1: Nat Biotechnol. 2007 Feb;25(2):221-31. Epub 2007 Jan 28.Click here to read Links
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Nat Biotechnol. 2007 Feb;25(2):189-90.

Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88.

DSM Food Specialties, PO Box 1, 2600 MA Delft, The Netherlands.

The filamentous fungus Aspergillus niger is widely exploited by the fermentation industry for the production of enzymes and organic acids, particularly citric acid. We sequenced the 33.9-megabase genome of A. niger CBS 513.88, the ancestor of currently used enzyme production strains. A high level of synteny was observed with other aspergilli sequenced. Strong function predictions were made for 6,506 of the 14,165 open reading frames identified. A detailed description of the components of the protein secretion pathway was made and striking differences in the hydrolytic enzyme spectra of aspergilli were observed. A reconstructed metabolic network comprising 1,069 unique reactions illustrates the versatile metabolism of A. niger. Noteworthy is the large number of major facilitator superfamily transporters and fungal zinc binuclear cluster transcription factors, and the presence of putative gene clusters for fumonisin and ochratoxin A synthesis.

PMID: 17259976 [PubMed - indexed for MEDLINE]