Abstract
Objective:
To study the prevalence of G1862T mutation in hepatitis B virus (HBV) isolates among Eastern Indian patients and its relationship with genotypes, HBeAg status and disease manifestation.
Methods:
HBV DNA was isolated from patients, amplified by nested PCR and sequenced directly.
Results:
Of the 102 patients, 32 were HBeAg positive and 70 HBeAg negative; 55, 24 and 23 isolates were infected with genotypes D, A and C, respectively. G1862T was detected in 18 samples, 15 (83%) of them belonged to genotype A (subgenotype HBV/A1), 3 (17%) to genotype D. This mutation was more frequent in HBeAg-negative than in HBeAg-positive patients (21 vs. 9%), whereas in HBV/A1 it was as common in HBeAg-positive as in HBeAg-negative patients and significantly associated with T1762/A1764 mutation. The mean viral load was lower in patients with G1862T mutation. Furthermore, this mutation was common in various clinical outcomes.
Conclusion:
In our community, G1862T mutation was predominantly found in HBV/A1 isolates irrespective of HBeAg status. Moreover this mutation could not be correlated to the clinical outcome. These findings indicate that the G1862T mutation is probably a part of the natural variability of HBV/A1.
(c) 2007 S. Karger AG, Basel.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adolescent
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Adult
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Aged
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Child
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Child, Preschool
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DNA, Viral / blood
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DNA, Viral / genetics*
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Female
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Genotype
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Hepatitis B / physiopathology
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Hepatitis B / virology*
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Hepatitis B e Antigens
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Hepatitis B virus / genetics*
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Hepatitis B virus / isolation & purification
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Hepatitis B virus / physiology
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Humans
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India
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Male
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Middle Aged
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Molecular Sequence Data
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Point Mutation*
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Polymerase Chain Reaction
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Sequence Analysis, DNA
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Viral Load
Substances
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DNA, Viral
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Hepatitis B e Antigens
Associated data
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GENBANK/AY274413
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GENBANK/AY274414
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GENBANK/AY274415
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GENBANK/AY274416
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