Syt VII KO animals exhibit impaired clearance of the intracellular pathogen
L. monocytogenes. Ag-specific T cell responses were primed in WT and Syt VII KO mice with 1 × 10
4 CFU of OVA-expressing recombinant
L. monocytogenes strain, JJL-OVA, or PBS alone. Ag-specific CD8 T cell responses were then evaluated 3 wk later following a secondary challenge with 2 × 10
5 CFU of JJL-OVA. Five days postsecondary challenge, Ag-specific CD8 T cell responses to the MHC class I-restricted OVA-derived peptide were examined in WT and Syt VII
−/− animals.
A, Ag-specific CD8
+ T cell in PBS (
top) and JJL-OVA-primed (
bottom) WT and Syt VII KO animals were determined following staining with CD11a FITC, CD8 PerCP, and control peptide- or SIINFEKL-loaded H-2 K
b-IgG dimer (the data shown are for CD8
+ -gated cells).
B, The average number of SIINFEKL-specific CD8
+ T cells in
A detected in the spleen 5 days postrechallenge in WT and Syt VII
−/− animals primed with PBS (
) or 1 × 10
4 CFU of JJL-OVA (■).
C, Splenic bacterial burden in the spleens of WT and Syt VII
−/− mice in
B were determined with splenic lysates on brain-heart infusion agar plates. The data are presented as the mean ± SEM for individual samples plated in triplicate in one representative experiment. The data in
A reflect representative data from three mice per group.*,
p >0.05 in paired Student’s
t test.