Relationship between DNA methylation and cell proliferation in Trypanosoma cruzi

M V Rojas; N Galanti

doi:10.1016/0014-5793(91)81377-k

Relationship between DNA methylation and cell proliferation in Trypanosoma cruzi

FEBS Lett. 1991 Dec 16;295(1-3):31-4. doi: 10.1016/0014-5793(91)81377-k.

Authors

M V Rojas¹, N Galanti

Affiliation

¹ Department of Cell Biology and Genetics, School of Medicine, University of Chile, Santiago.

PMID: 1722465
DOI: 10.1016/0014-5793(91)81377-k

Abstract

5-Azacytidine treatment of T. cruzi epimastigotes in culture induces active cell proliferation. This effect was detected as an increase in the cell number and [3H-methyl]thymidine incorporation into DNA. 5-Azacytidine does not alter other metabolic parameters. We have previously demonstrated that 5-azacytidine induces DNA hypomethylation in T. cruzi. Accordingly, we suggest that this chemical modification may be related to the control of T. cruzi cell division.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Azacitidine / pharmacology*
Cell Division / drug effects
Cells, Cultured
DNA
DNA Replication / drug effects
DNA, Protozoan / drug effects
DNA, Protozoan / metabolism*
Kinetics
Methylation
Oxygen Consumption / drug effects
Thymidine / metabolism
Trypanosoma cruzi / cytology
Trypanosoma cruzi / drug effects
Trypanosoma cruzi / physiology*

Substances

DNA, Protozoan
DNA
Azacitidine
Thymidine