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1: FEBS Lett. 2007 Jan 23;581(2):337-41. Epub 2007 Jan 11.Click here to read Links

A Turnip yellow mosaic virus infection system in Arabidopsis suspension cell culture.

Camborde L, Tournier V, Noizet M, Jupin I.

Laboratoire de Virologie Moléculaire, Institut Jacques Monod, UMR 7592 CNRS, Universités Paris 6, Paris 7, 2 place Jussieu, 75251 Paris Cedex 05, France.

Turnip yellow mosaic virus (TYMV) is a positive-strand RNA virus able to infect Arabidopsis thaliana. To establish a TYMV infection system in Arabidopsis cell culture, TYMV replicons with the capsid protein gene replaced by a reporter gene expressing the Sh ble protein conferring zeocin resistance were used to transfect Arabidopsis cells. Zeocin-resistant Arabidopsis calli were used to generate a suspension cell culture. Detection of viral proteins and RNAs after 18 months in culture demonstrated persistent replication of the replicon. The Arabidopsis cell culture yielded soluble, active replication complexes, providing a useful tool to study host factors involved in TYMV replication.

PMID: 17222410 [PubMed - indexed for MEDLINE]

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