Increased numbers of CNS-infiltrating inflammatory Th17 cells in il25^−/− mice. (A) Intracellular cytokine staining of FITC-gated CD4⁺ T cells isolated from the CNS of mice at day 7 after immunization (left, flow cytometric analysis; right, calculated number of cytokine-producing cells). (B) Number of cytokine-producing CD4⁺ cells in CNS taken at peak of disease. For A and B, cells from four mice were pooled for intracellular cytokine analysis. Data are representative of two experiments. (C) Disease score of il25^−/− mice immunized with MOG/CFA (n = 4 mice per group) and treated with antibodies against IL-17 or IFNγ or with rat IgG1 control antibody. Data shown are representative of two experiments.

IL-25 inhibits the effector phase of EAE and suppresses clinical disease relapse. (A) Clinical score of SJL mice (n = 5 per group) injected with 3 × 10⁹ particles of indicated rAdV by intracerebral ventricular injection 1 d before or 10 d after immunization. (B) Histopathological analysis of spinal cords from SJL mice at day 18 after immunization. Mice were treated with GFP-rAdV (left) or IL-25-rAdV (right) at day 1. (bottom) 10-fold magnification of boxed area in top panels. Bars, 100 μm. Results of histological analysis are summarized in Table S1 (available at http://www.jem.org/cgi/content/full/jem.20061738/DC1). (C) Clinical score of mice (n = 4–5 per group) that received 2 × 10⁷ activated PLP-specific lymphocytes. Recipient mice were treated with 3 × 10⁹ particles of indicated rAdV at day 0 (top) or day 3 (bottom) after PLP-cell adoptive transfer. (left) Kinetics of mean disease score; (right) maximum disease score per individual mouse during 16 d of observation. (D) Clinical score of EAE-primed mice (n = 7–10 per group) injected with 3 × 10⁹ particles of indicated rAdV by an intracerebral ventricular route during disease remission (20 d after immunization, arrow). Mice were observed for two disease-relapse cycles. Results are representative of at least two experiments.

IL-13 is required for Th17 suppression in IL-25–mediated protection from EAE. Clinical score of il4^−/− (A), il13^−/− (B), or il4ra^−/− mice (C), with five mice per group injected daily with PBS or mIL-25 (s.c.) from days 1–12. Results are representative of two experiments. (D–F) Naive T cells and LPS-activated CD11c⁺ DCs were obtained from DO11.10 × RAG^−/− mice (pool of five mice) and incubated for 3 d with IL-23 in the presence of OVA peptide. IFNγ and IL-4 were neutralized in these cultures. (D) The effect of IL-25 addition on the number of IL-23–induced IL-17 producers was assessed by intracellular staining for IL-17. (E) IL-13 expression in these cultures was determined by LUMINEX. (F) The modulation of IL-17 protein production in IL-23–driven cultures by IL-25, IL-25 plus anti–IL-13, and IL-13 is shown in percentage of IL-23–induced IL-17 production without addition of exogenous factors (mean IL-17 production is 202.3 pg/ml, assessed by LUMINEX). Data shown are representative of at least two experiments (D and E) or pooled data from two similar experiments (F). (G) Purified MOG_33-55 TCR transgenic T cells and LPS-activated CD11c⁺ DCs were incubated for 4 d with IL-23 in the presence of MOG peptide and neutralizing antibodies against IFNγ and IL-4. IL-17 production after incubation with IL-25, IL-25 plus anti–IL-13, and IL-13 was measured by LUMINEX. (H) DCs were purified from spleens and superficial lymph nodes and activated with LPS with or without addition of IL-13. After 24 h, mRNA expression relative to ubiquitin was measured for IL-23p19, IL-1β, and IL-6 by quantitative real-time PCR. Shown is an experiment using DCs from C57BL/6 mice representative of two C57BL/6 and two BALB/c experiments with a similar outcome (n = 3–5 mice pooled per experiment).

il25^−/− mice are highly susceptible to EAE. (A) Taqman PCR analysis of IL-25 mRNA levels in spinal cords from C57BL/6 WT normal and diseased versus il25-LacZ-KI normal and diseased (n = 6 mice per group). Data shown are representative of at least two experiments. ND, not detectable. (B) IL-25 mRNA expression in CNS-resident microglia. Brain leukocytes were isolated from bone marrow chimeras expressing the CD45.2 allotype on radiation-resistant host cells and the CD45.1 allotype on donor leukocytes. CD11b⁺CD45.2^loCD45.1^neg resident microglia were purified from >100 irradiation bone marrow chimeric naive or diseased (day 14) mice. Data from quantitative real-time PCR analysis for IL-25 expression were normalized to ubiquitin. (C) Clinical score (top) and percentage of incidence of disease (bottom) of C57BL/6 (WT) and il25^−/− mice immunized with MOG/CFA s.c. on day 0 and PTX i.v. on days 0 and 2. The dagger indicates that by day 17, all il25^−/− mice had died from EAE-associated complications. Data shown are 7–9 mice per group and are representative of at least three experiments.

Reduced Th2 cytokine production but normal regulatory T cell response in il25^−/− DLN cells. (A) mRNA expression in MOG-stimulated DLN cells taken at day 7 after immunization. Expression levels are normalized to ubiquitin. n = 5. Obtained DLN samples were pooled in three different pools for mRNA processing. (B) In vitro cytokine recall response. DLN cells from MOG-immunized il25^−/− and WT mice were isolated at day 7 after immunization. Cells were stimulated with MOG peptide for 3 d and then restimulated with anti-CD3/anti-CD28 for 2 additional days. Supernatants were from 24- or 72-h cultures as indicated. Data are representative of three experiments. All cytokines were measured by cytometric bead array except for IL-13 and IL-17, which were detected by ELISA as described in Materials and methods. ND, not detectable. *, P < 0.05 (unpaired two-tailed t test). (C) Generation of regulatory T cells in il25^−/− and WT mice before (pooled sample of two mice per group) and 7 d after (pooled sample of four mice per group) immunization with MOG/CFA. DLN cells were stained for CD4, CD25, and Foxp3.

IL-25 induces Th2 and suppresses Th17 independent of IFNγ. (A) Clinical score of immunized WT and il25^−/− mice compared with immunized WT mice injected daily s.c. with mIL-25 protein from day 1 to the end of the experiment. Results are representative for at least four experiments with five to six mice per group. (B) Clinical score of ifng^−/− (GKO) mice (n = 5 per group) injected daily with PBS or mIL-25 (s.c.) from days 1–12. Results are representative of two experiments. (C) DLN cells from MOG-immunized WT or ifng^−/− mice treated with IL-25 or saline were analyzed for cytokine gene expression at day 7 after immunization (five mice per group; DLN cells were processed in three different pools for mRNA analysis). Cytokine gene expression was normalized to ubiquitin. (D) In vitro cytokine recall response. DLN cells from MOG-immunized WT mice treated with PBS or mIL-25 daily s.c. were isolated at day 7 after immunization. Cells were stimulated with MOG peptide for 3 d and restimulated with anti-CD3/anti-CD28 for 2 additional days. Cytokine levels in supernatants were measured by CBA or ELISA. Cells from individual animals were cultured separately, and data are mean cytokine levels ± SD of three to five mice per treatment group. For IFNγ detection, nine mice per treatment group are shown. Data are representative of at least two experiments. *, P < 0.05. (E) DLN cells from MOG-immunized WT and ifng^−/− (GKO) mice treated with IL-25 or saline (pool of four mice per group) were stimulated in vitro with MOG for 3 d followed by incubation with IL-2 for another 2 d. Frequencies (FACS plots) and absolute numbers (bar graph) of MOG-specific IL-17–producing CD4⁺ cells were assessed. Data shown are representative of two experiments.