Effect of cell-capillary tip distance and pulse duration. (A) Average fluorescence intensity (normalized) vs. time plotted for all cells electroporated with a single pulse of 200 ms and cell-capillary tip distance of 2.0 μm − 10.0 μm at 500 V. The pink, green, blue, red and black curves correspond to 10.0, 7.0, 5.0, 3.5, and 2.0 μm cell-capillary tip distances respectively. (B) Average fluorescence intensity (normalized) vs. time plotted for all cell electroporated with a single pulse of varying pulse durations (50 ms to 300 ms), and cell-capillary tip distance of 3.5 μm at 500 V. Black, red, blue, and green curves correspond to 50, 100, 200, and 300 ms pulse durations respectively. All the data were corrected for bleaching.