Schistosoma mansoni: the IMP4 gene is involved in DNA repair/tolerance after treatment with alkylating agent methyl methane sulfonate

Carolina Furtado; Carlos Gustavo Regis-da-Silva; Danielle Gomes Passos-Silva; Glória Regina Franco; Andréa Mara Macedo; Sérgio Danilo Junho Pena; Carlos Renato Machado

doi:10.1016/j.exppara.2006.11.001

Schistosoma mansoni: the IMP4 gene is involved in DNA repair/tolerance after treatment with alkylating agent methyl methane sulfonate

Exp Parasitol. 2007 May;116(1):25-34. doi: 10.1016/j.exppara.2006.11.001. Epub 2006 Dec 26.

Authors

Carolina Furtado¹, Carlos Gustavo Regis-da-Silva, Danielle Gomes Passos-Silva, Glória Regina Franco, Andréa Mara Macedo, Sérgio Danilo Junho Pena, Carlos Renato Machado

Affiliation

¹ Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, CP 486, 31270-010 Belo Horizonte, Minas Gerais, Brazil.

PMID: 17189633
DOI: 10.1016/j.exppara.2006.11.001

Abstract

Using a functional complementation strategy, we have isolated a Schistosoma mansoni cDNA that complemented Escherichia coli mutant strains which are defective in the DNA base excision repair pathway. This cDNA partially complemented the MMS-sensitive phenotype of these strains. The sequence of the isolated cDNA was homologous to genes involved in the RNA metabolism pathway, especially ScIMP4 of Saccharomyces cerevisiae. To establish whether the S. mansoni cDNA clone could complement yeast ScIMP4-defective mutants, we constructed a yeast haploid strain that coded for a truncated Imp4p protein. This mutant strain was treated with different DNA damaging agents, but showed only MMS sensitivity. The functional homology between the ScIMP4 gene and the cDNA from S. mansoni was verified by partial complementation of the mutant yeast with the worm's gene. This gene appears to be involved in DNA repair and RNA metabolism in both S. mansoni and S. cerevisiae.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alkylating Agents / pharmacology*
Amino Acid Sequence
Animals
Base Sequence
DNA Repair / genetics*
DNA, Complementary / chemistry
Escherichia coli / drug effects
Escherichia coli / genetics
Escherichia coli / radiation effects
Gene Library
Genetic Complementation Test
Hydroxyurea / pharmacology
Methyl Methanesulfonate / pharmacology*
Molecular Sequence Data
Mutation
Nucleic Acid Synthesis Inhibitors / pharmacology
Phenotype
Polymerase Chain Reaction
Ribosomal Proteins / chemistry
Ribosomal Proteins / genetics*
Ribosomal Proteins / physiology
Saccharomyces cerevisiae / drug effects
Saccharomyces cerevisiae / genetics
Saccharomyces cerevisiae / radiation effects
Saccharomyces cerevisiae Proteins / chemistry
Saccharomyces cerevisiae Proteins / genetics*
Saccharomyces cerevisiae Proteins / physiology
Schistosoma mansoni / drug effects
Schistosoma mansoni / genetics*
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid

Substances

Alkylating Agents
DNA, Complementary
IMP4 protein, S cerevisiae
Nucleic Acid Synthesis Inhibitors
Ribosomal Proteins
Saccharomyces cerevisiae Proteins
Methyl Methanesulfonate
Hydroxyurea