Vaccine. 2007 Feb 19;25(9):1647-57. Epub 2006 Nov 17.

Production of Escherichia coli heat labile toxin (LT) B subunit in soybean seed and analysis of its immunogenicity as an oral vaccine.

Moravec T, Schmidt MA, Herman EM, Woodford-Thomas T.

Source

Donald Danforth Plant Science Center, 975 North Warson Road, St. Louis, MO 63132, United States.

Abstract

The B subunit of the heat labile toxin of enterotoxigenic Escherichia coli (LTB) was used as a model immunogen for production in soybean seed. LTB expression was directed to the endoplasmic reticulum (ER) of seed storage parenchyma cells for sequestration in de novo synthesized inert protein accretions derived from the ER. Pentameric LTB accumulated to 2.4% of the total seed protein at maturity and was stable in desiccated seed. LTB-soybean extracts administered orally to mice induced both systemic IgG and IgA, and mucosal IgA antibody responses, and was particularly efficacious when used in a parenteral prime-oral gavage boost immunization strategy. Sera from immunized mice blocked ligand binding in vitro and immunized mice exhibited partial protection against LT challenge. Moreover, soybean-expressed LTB stimulated the antibody response against a co-administered antigen by 500-fold. These results demonstrate the utility of soybean as an efficient production platform for vaccines that can be used for oral delivery.

PMID:: 17188785; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

Publication Types

Research Support, Non-U.S. Gov't

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Supplemental Content

Save items

Related citations in PubMed

Expression and immunogenicity of enterotoxigenic Escherichia coli heat-labile toxin B subunit in transgenic rice callus. [Mol Biotechnol. 2010]
Expression and immunogenicity of enterotoxigenic Escherichia coli heat-labile toxin B subunit in transgenic rice callus.
Kim TG, Kim BG, Kim MY, Choi JK, Jung ES, Yang MS. Mol Biotechnol. 2010 Jan; 44(1):14-21. Epub 2009 Aug 6.
Functional pentameric formation via coexpression of the Escherichia coli heat-labile enterotoxin B subunit and its fusion protein subunit with a neutralizing epitope of ApxIIA exotoxin improves the mucosal immunogenicity and protection against challenge by Actinobacillus pleuropneumoniae. [Clin Vaccine Immunol. 2011]
Functional pentameric formation via coexpression of the Escherichia coli heat-labile enterotoxin B subunit and its fusion protein subunit with a neutralizing epitope of ApxIIA exotoxin improves the mucosal immunogenicity and protection against challenge by Actinobacillus pleuropneumoniae.
Kim JM, Park SM, Kim JA, Park JA, Yi MH, Kim NS, Bae JL, Park SG, Jang YS, Yang MS, et al. Clin Vaccine Immunol. 2011 Dec; 18(12):2168-77. Epub 2011 Oct 26.
Evaluation of different promoter sequences and antigen sorting signals on the immunogenicity of Bacillus subtilis vaccine vehicles. [Vaccine. 2007]
Evaluation of different promoter sequences and antigen sorting signals on the immunogenicity of Bacillus subtilis vaccine vehicles.
Paccez JD, Nguyen HD, Luiz WB, Ferreira RC, Sbrogio-Almeida ME, Schuman W, Ferreira LC. Vaccine. 2007 Jun 11; 25(24):4671-80. Epub 2007 Apr 25.
Review Analysis of strategies to successfully vaccinate infants in developing countries against enterotoxigenic E. coli (ETEC) disease. [Vaccine. 2007]
Review Analysis of strategies to successfully vaccinate infants in developing countries against enterotoxigenic E. coli (ETEC) disease.
Walker RI, Steele D, Aguado T, Ad Hoc ETEC Technical Expert Committee. Vaccine. 2007 Mar 30; 25(14):2545-66. Epub 2006 Dec 19.
Review Targeting of plant-derived vaccine antigens to immunoresponsive mucosal sites. [Vaccine. 2003]
Review Targeting of plant-derived vaccine antigens to immunoresponsive mucosal sites.
Rigano MM, Sala F, Arntzen CJ, Walmsley AM. Vaccine. 2003 Jan 30; 21(7-8):809-11.

See reviews... See all...

Cited by 7 PubMed Central articles

Silencing of soybean seed storage proteins results in a rebalanced protein composition preserving seed protein content without major collateral changes in the metabolome and transcriptome. [Plant Physiol. 2011]
Silencing of soybean seed storage proteins results in a rebalanced protein composition preserving seed protein content without major collateral changes in the metabolome and transcriptome.
Schmidt MA, Barbazuk WB, Sandford M, May G, Song Z, Zhou W, Nikolau BJ, Herman EM. Plant Physiol. 2011 May; 156(1):330-45. Epub 2011 Mar 10.
Expression of antibody fragments with a controlled N-glycosylation pattern and induction of endoplasmic reticulum-derived vesicles in seeds of Arabidopsis. [Plant Physiol. 2011]
Expression of antibody fragments with a controlled N-glycosylation pattern and induction of endoplasmic reticulum-derived vesicles in seeds of Arabidopsis.
Loos A, Van Droogenbroeck B, Hillmer S, Grass J, Pabst M, Castilho A, Kunert R, Liang M, Arcalis E, Robinson DG, et al. Plant Physiol. 2011 Apr; 155(4):2036-48. Epub 2011 Feb 16.
Review Plant-made vaccine antigens and biopharmaceuticals. [Trends Plant Sci. 2009]
Review Plant-made vaccine antigens and biopharmaceuticals.
Daniell H, Singh ND, Mason H, Streatfield SJ. Trends Plant Sci. 2009 Dec; 14(12):669-79. Epub 2009 Oct 14.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Production of Escherichia coli heat labile toxin (LT) B subunit in soybean seed ...
Production of Escherichia coli heat labile toxin (LT) B subunit in soybean seed and analysis of its immunogenicity as an oral vaccine.
Vaccine. 2007 Feb 19 ;25(9):1647-57. Epub 2006 Nov 17 .

PubMed
Stimulatory effect of oral administration of tea, coffee or caffeine on UVB-indu...
Stimulatory effect of oral administration of tea, coffee or caffeine on UVB-induced apoptosis in the epidermis of SKH-1 mice.
Toxicol Appl Pharmacol. 2007 Nov 1 ;224(3):209-13. Epub 2006 Nov 7 .

PubMed
Angiogenesis and improved cerebral blood flow in the ischemic boundary area dete...
Angiogenesis and improved cerebral blood flow in the ischemic boundary area detected by MRI after administration of sildenafil to rats with embolic stroke.
Brain Res. 2007 Feb 9 ;1132(1):185-92. Epub 2006 Dec 26 .

PubMed
The dual actions of angiogenesis and anti-apoptosis induced by an isolated fract...
The dual actions of angiogenesis and anti-apoptosis induced by an isolated fraction from Geum japonicum repair muscle ischemia.
Arch Biochem Biophys. 2007 Mar 1 ;459(1):91-7. Epub 2006 Nov 7 .

PubMed
Bone mineral density in children and adolescents with neurofibromatosis type 1.
Bone mineral density in children and adolescents with neurofibromatosis type 1.
J Pediatr. 2007 Jan ;150(1):83-8.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: