Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Biosens Bioelectron. 2007 Jun 15;22(12):2834-40. Epub 2006 Dec 21.

Label-free detection of single nucleotide polymorphisms utilizing the differential transfer function of field-effect transistors.

Author information

  • 1Institute of Bio- and Nanosystems, Institute 2: Bioelectronics, Center of Nanoelectronic Systems for Information Technology, Forschungszentrum Jülich GmbH, D-52425 Jülich, Germany. s.ingebrandt@fz-juelich.de

Abstract

We present a label-free method for the detection of DNA hybridization, which is monitored by non-metallized silicon field-effect transistors (FET) in a microarray approach. The described method enables a fast and fully electronic readout of ex situ binding assays. The label-free detection utilizing the field-effect is based on the intrinsic charge of the DNA molecules and/or on changes of the solid-liquid interface impedance, when biomolecules bind to the sensor surface. With our sensor system, usually a time-resolved, dc readout is used. In general, this FET signal suffers from sensor drift, temperature drift, changes in electrolyte composition or pH value, influence of the reference electrode, etc. In this article, we present a differential ac readout concept for FET microarrays, which enables a stable operation of the sensor against many of these side-parameters, reliable readout and a possibility for a quick screening of large sensor arrays. We present the detection of point mutations in short DNA samples with this method in an ex situ binding assay.

PMID:
17187976
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk