Science. 2006 Dec 22;314(5807):1893.

Untemplated oligoadenylation promotes degradation of RISC-cleaved transcripts.

Ibrahim F, Rohr J, Jeong WJ, Hesson J, Cerutti H.

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Biological Sciences and Plant Science Initiative, University of Nebraska, Lincoln, NE 68588, USA.

Abstract

Double-stranded RNA, processed to small interfering RNAs (siRNAs) by Dicer and incorporated into the RNA-induced silencing complex (RISC), triggers gene silencing by a variety of pathways in eukaryotes. RNA interference involving the degradation of homologous transcripts is the best-characterized mechanism. However, the fate of the RNA fragments resulting from siRNA-directed cleavage is poorly understood. We have identified a gene (MUT68) in the unicellular green alga Chlamydomonas reinhardtii that is required for the efficient decay of siRNA-targeted transcripts. MUT68 encodes a noncanonical polyadenylate polymerase that adds untemplated adenines to the 5' RNA fragments after siRNA-mediated cleavage and appears to stimulate their exosome-dependent degradation.

PMID:: 17185594; [PubMed - indexed for MEDLINE]

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