Biochem Biophys Res Commun. 1991 Oct 15;180(1):342-8.

High intracellular pH in CFPAC: a pancreas cell line from a patient with cystic fibrosis is lowered by retrovirus-mediated CFTR gene transfer.

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Department of Comparative Medicine, University of Alabama, Birmingham School of Medicine 35294.

Abstract

Expression of CFTR from a retroviral vector in CFPAC, a pancreatic adenocarcinoma cell line derived from a patient with Cystic Fibrosis, causes a decrease in the average intracellular pH (pHi) in these transduced clones (PLJ-CFTR), as compared to CFPAC or CFPAC transduced with control virus (PLJ clones). Whereas the average pHi calculated based on results obtained in two PLJ-CFTR clones, PLJ-CFTR-20 (n = 2) and PLJ-CFTR-6 (n = 5), was 7.46 +/- 0.07, the average pHi calculated from results obtained in CFPAC (n = 13), PLJ-6 (n = 11) and PLJ-10 (n = 3) was pH 7.83 +/- 0.11. This finding suggests that CFTR may be involved, directly or indirectly, in the regulation of pHi in the pancreas.

PMID:: 1718277; [PubMed - indexed for MEDLINE]

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