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Definition of surface-exposed epitopes on the (Ca(2+)-Mg2+)-ATPase of sarcoplasmic reticulum.
Department of Biochemistry, University of Southampton, U.K.
Epitopes for monoclonal antibodies binding to the native (Ca(2+)-Mg2+)-ATPase have been defined by studying binding to sets of hexameric peptides synthesized on plastic pegs. Epitopes have been confirmed by demonstrating the binding of anti-peptide antibodies to the ATPase. A method is presented for definition of surface-exposed epitopes using polyclonal antibodies. Three surface-exposed epitopes have been defined in the nucleotide-binding domain of the ATPase, suggesting considerable surface exposure of this region. Other surface-exposed epitopes have been located in the region of the fourth stalk domain.
PMID: 1718260 [PubMed - indexed for MEDLINE]
PMCID: PMC1151567
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Cited by 5 PubMed Central articles
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Sarco/endoplasmic-reticulum calcium ATPase SERCA1 is maintained in the endoplasmic reticulum by a retrieval signal located between residues 1 and 211.
Newton T, Black JP, Butler J, Lee AG, Chad J, East JM.
Biochem J. 2003 May 1; 371(Pt 3):775-82.
[Biochem J. 2003]
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Thapsigargin-sensitive Ca(2+)-ATPases account for Ca2+ uptake to inositol 1,4,5-trisphosphate-sensitive and caffeine-sensitive Ca2+ stores in adrenal chromaffin cells.
Poulsen JC, Caspersen C, Mathiasen D, East JM, Tunwell RE, Lai FA, Maeda N, Mikoshiba K, Treiman M.
Biochem J. 1995 May 1; 307 ( Pt 3):749-58.
[Biochem J. 1995]
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Identification of the amino acids comprising a surface-exposed epitope within the nucleotide-binding domain of the Na+,K(+)-ATPase using a random peptide library.
Malik B, Jamieson GA Jr, Ball WJ Jr.
Protein Sci. 1993 Dec; 2(12):2103-11.
[Protein Sci. 1993]
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