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Carbohydr Res. 2007 Feb 5;342(2):274-8. Epub 2006 Nov 29.

Structural determination of the O-antigenic polysaccharide from Escherichia coli O166.

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  • 1Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, and Karolinska Institute, Department of Laboratory Medicine, Division of Clinical Bacteriology, Karolinska University Hospital, S-141 86 Stockholm, Sweden.

Abstract

The O-antigen of the lipopolysaccharide from Escherichia coli O166 has been determined by component analysis together with 1D and 2D NMR spectroscopy techniques. The polysaccharide has pentasaccharide repeating units consisting of D-glucose (1), D-galactose (2) and N-acetyl-D-galactosamine (2) with the following structure: [

STRUCTURE:

SEE TEXT]. In the 1H NMR, spectrum resonances of low intensity were observed. Further analysis of these showed that they originate from the terminal part of the polysaccharide, thereby revealing that the repeating unit has a 3-substituted N-acetyl-D-galactosamine residue at its reducing end.

[PubMed - indexed for MEDLINE]
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