J Immunol. 1991 Nov 1;147(9):3128-32.

N-glycosylation of HIV-gp120 may constrain recognition by T lymphocytes.

Botarelli P, Houlden BA, Haigwood NL, Servis C, Montagna D, Abrignani S.

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Department of Allergy/Immunology, Ciba-Geigy Ltd, Basel, Switzerland.

Abstract

The HIV envelope protein gp120 is heavily glycosylated, having 55% of its molecular mass contributed by N-linked carbohydrates. We investigated the role of N-glycosylation in presentation of HIV-gp120 to T cells. T cell clones obtained from humans immunized with a recombinant nonglycosylated form of HIV-gp120 (env 2-3) were studied for their ability to recognize both env 2-3 and glycosylated gp120. We found that 20% of CD4+ T cell clones specific for env 2-3 fail to respond to glycosylated gp120 of the same HIV isolate. Using synthetic peptides, we mapped one of the epitopes recognized by such clones to the sequence 292-300 (NESVAINCT), which contains two asparagines that are glycosylated in the native gp120. These findings suggest that N-linked carbohydrates within an epitope can function as hindering structures that limit Ag recognition by T lymphocytes.

PMID:: 1717587; [PubMed - indexed for MEDLINE]

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