Format

Send to

Choose Destination
See comment in PubMed Commons below
Clin Chim Acta. 2007 Mar;378(1-2):105-11. Epub 2006 Nov 10.

Establishment and evaluation of 2 monoclonal antibodies against oxidized apolipoprotein A-I (apoA-I) and its application to determine blood oxidized apoA-I levels.

Author information

  • 1Ikagaku Co. Ltd., Furukawacho, 328 Hazukashi Fushimi-ku, Kyoto 612-8486, Japan. ueda@ikagaku.co.jp

Abstract

BACKGROUND:

Apolipoprotein A-I (apoA-I) is the major lipoprotein component of high-density lipoprotein(HDL), and plays an important role in reverse cholesterol transport. Its function is known to be influenced by oxidation.

METHODS:

Using H2O2-or chloramine T-oxidized apoA-I as antigen, we prepared 2 kinds of monoclonal antibodies, and established an ELISA system for the measurement of oxidized apoA-I.

RESULTS:

The 2 monoclonal antibodies obtained, 7D3 and 98A7, exhibited different reactivity characteristics. The serum level of oxidized apoA-I was higher in patients with either inflammatory disease or diabetes than in healthy individuals, and suggested a diversity of oxidized apoA-I.

CONCLUSION:

The 2 monoclonal antibodies are useful for the determination of oxidized apoA-I and study of diverse oxidized HDLs.

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk