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Methods Mol Biol. 2007;360:155-61.

Production of siRNA- and cDNA-transfected cell arrays on noncoated chambered coverglass for high-content screening microscopy in living cells.

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  • 1Cell Biology and Cell Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.


In this chapter, we provide a protocol for the production of transfected cell arrays in living mammalian cells on noncoated chambered coverglass for the systematic functional analyses of human genes by high-content screening microscopy. This method should facilitate drug target validation by small-interfering RNAs.

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