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J Fluoresc. 2007 Jan;17(1):43-8. Epub 2006 Dec 14.

Fluorescence lifetime correlation spectroscopy.

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  • 1PicoQuant GmbH, Rudower Chaussee 29, 12489 Berlin, Germany.


This article explains the basic principles of FLCS, a genuine fusion of Time-Correlated Single Photon Counting (TCSPC) and Fluorescence Correlation Spectroscopy (FCS), using common terms and minimum mathematics. The usefulness of the method is demonstrated on simple FCS experiments. The method makes possible to separate the autocorrelation function of individual components of a mixture of fluorophores, as well as purging the result from parasitic contributions like scattered light or detector afterpulsing.

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