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J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Aug;855(1):51-8. Epub 2006 Dec 11.

Analysis of DNA-bound advanced glycation end-products by LC and mass spectrometry.

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  • 1Institute of Pharmacy and Food Chemistry, University of Erlangen-Nuremberg, Erlangen, Germany.

Abstract

Sugars and sugar degradation products readily react in vitro with guanine derivatives, resulting in the formation of DNA-bound advanced glycation end-products (DNA-AGEs). The two diastereomers of N(2)-(1-carboxyethyl)-2'-deoxyguanosine (CEdG(A,B)) and the cyclic adduct of methylglyoxal and 2'-deoxyguanosine (mdG) (N(2)-7-bis(1-hydroxy-2-oxopropyl)-2'-deoxyguanosine have also been detected in cultured cells and/or in vivo. LC-MS/MS methods have been developed to analyze sensitively DNA adducts in vitro and in vivo. In this paper, the chemical structures of possible DNA-AGEs and the application of LC-MS/MS to measure DNA-AGEs are reviewed.

PMID:
17161667
[PubMed - indexed for MEDLINE]
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