Nucleic Acids Symp Ser (Oxf). 2005;(49):123-4.

SNPs analysis by use of oligonucleotides containing 2'-O-methyl-2-thiouridines.

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Graduate School of Life Science, Tokyo Institute of Technology.

Abstract

In this study, we report an improved method for the synthesis of oligonucleotides containing 2-thiouridine (s2U) derivatives. The hybridization affinity and base pair recognition ability of oligonucleotides having 2-thiouracil moieties with both the complementary RNAs and DNAs were also studied in detail. These results indicate that oligonucleotides containing s2U derivatives could be used as powerful tools for chip-based SNPs detection.

PMID:: 17150664; [PubMed - indexed for MEDLINE]

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