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    Biochem Biophys Res Commun. 1991 Aug 15;178(3):1386-92.

    Molecular cloning of the human histamine H2 receptor.

    Gantz I, Munzert G, Tashiro T, Schäffer M, Wang L, DelValle J, Yamada T.

    Department of Surgery, University of Michigan Medical Center, Ann Arbor.

    We utilized the technique of polymerase chain reaction with oligonucleotide primers based upon the nucleotide sequence of the canine H2 histamine receptor gene which we recently isolated to clone its human homologue. Transfection of a construct of this gene in Colo-320 DM cells led to the expression of a receptor that bound to [methyl-3H] tiotidine and was linked to 3',5'cyclic adenosine monophosphate (cAMP) generation in response to histamine. Both cAMP generation and [methyl-3H] tiotidine binding were inhibited with the H2 histamine receptor selective antagonist cimetidine but not diphenhydramine or thioperamide which are, respectively, H1 and H3 histamine receptor antagonists. These data confirm that we have successfully cloned a novel gene encoding the human H2 histamine receptor.

    PMID: 1714721 [PubMed - indexed for MEDLINE]

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    • Cimetidine (Tagamet®, Tagamet® HB, Tagamet® Tiltab®)

      Cimetidine is used to treat ulcers; gastroesophageal reflux disease (GERD), a condition in which backward flow of acid from the stomach causes heartburn and injury of the food pipe (esophagus); and conditions where the s...

    • Diphenhydramine (AllerMax®, AllerMax® Caplets®, Benadryl®, ...)

      Diphenhydramine is used to relieve red, irritated, itchy, watery eyes; sneezing; and runny nose caused by hay fever, allergies, or the common cold. Diphenhydramine is also used to relieve cough caused by minor throat or ...

    • Source: AHFS Consumer Medication Information

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