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Anal Biochem. 1991 Mar 2;193(2):225-30.

Direct detection of yeast mutants with reduced viability on plates by erythrosine B staining.

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  • 1Laboratoire de Génétique, Université de Bordeaux II, Talence, France.

Abstract

In this paper we report a rapid method to screen yeast mutants exhibiting reduced viability directly on plates. This method avoids the need for replica plating and is based on the addition of the vital dye erythrosine B in nutrient medium. After 2 or 3 days of culture, colonies containing a large proportion of dead cells show a pink or a dark pink color whereas normal colonies are practically white.

PMID:
1714683
[PubMed - indexed for MEDLINE]
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