Nat Rev Mol Cell Biol. 2006 Dec;7(12):952-8.

Functional and quantitative proteomics using SILAC.

Source

Department of Proteomics and Signal Transduction, Max-Planck Institute for Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany. mmann@biochem.mpg.de

Abstract

Researchers in many biological areas now routinely characterize proteins by mass spectrometry. Among the many formats for quantitative proteomics, stable-isotope labelling by amino acids in cell culture (SILAC) has emerged as a simple and powerful one. SILAC removes false positives in protein-interaction studies, reveals large-scale kinetics of proteomes and - as a quantitative phosphoproteomics technology - directly uncovers important points in the signalling pathways that control cellular decisions.

PMID:: 17139335; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

Publication Types

Research Support, Non-U.S. Gov't

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Other Literature Sources

Supplemental Content

Save items

Related citations in PubMed

Stable isotope labeling by amino acids in cell culture for quantitative proteomics. [Methods Mol Biol. 2007]
Stable isotope labeling by amino acids in cell culture for quantitative proteomics.
Ong SE, Mann M. Methods Mol Biol. 2007; 359:37-52.
Quantitative proteomics using stable isotope labeling with amino acids in cell culture. [Nat Protoc. 2008]
Quantitative proteomics using stable isotope labeling with amino acids in cell culture.
Harsha HC, Molina H, Pandey A. Nat Protoc. 2008; 3(3):505-16.
Stable isotope labeling by amino acids in cell culture (SILAC). [Methods Mol Biol. 2008]
Stable isotope labeling by amino acids in cell culture (SILAC).
Gruhler S, Kratchmarova I. Methods Mol Biol. 2008; 424:101-11.
Review [Quantitative proteomics by SILAC: practicalities and perspectives for an evolving approach]. [Med Sci (Paris). 2009]
Review [Quantitative proteomics by SILAC: practicalities and perspectives for an evolving approach].
Emadali A, Gallagher-Gambarelli M. Med Sci (Paris). 2009 Oct; 25(10):835-42.
Review Use of stable isotope labeling by amino acids in cell culture (SILAC) for phosphotyrosine protein identification and quantitation. [Methods Mol Biol. 2009]
Review Use of stable isotope labeling by amino acids in cell culture (SILAC) for phosphotyrosine protein identification and quantitation.
Zhang G, Neubert TA. Methods Mol Biol. 2009; 527:79-92, xi.

See reviews... See all...

Cited by 88 PubMed Central articles

Proteomic technologies for the study of osteosarcoma. [Sarcoma. 2012]
Proteomic technologies for the study of osteosarcoma.
Byrum SD, Washam CL, Montgomery CO, Tackett AJ, Suva LJ. Sarcoma. 2012; 2012:169416. Epub 2012 Mar 6.
Cardioproteomics: advancing the discovery of signaling mechanisms involved in cardiovascular diseases. [Am J Cardiovasc Dis. 2011]
Cardioproteomics: advancing the discovery of signaling mechanisms involved in cardiovascular diseases.
Cui Z, Dewey S, Gomes AV. Am J Cardiovasc Dis. 2011; 1(3):274-92. Epub 2011 Sep 10.
Antibody-based protein profiling of the human chromosome 21. [Mol Cell Proteomics. 2012]
Antibody-based protein profiling of the human chromosome 21.
Uhlén M, Oksvold P, Älgenäs C, Hamsten C, Fagerberg L, Klevebring D, Lundberg E, Odeberg J, Pontén F, Kondo T, et al. Mol Cell Proteomics. 2012 Mar; 11(3):M111.013458. Epub 2011 Oct 31.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Functional and quantitative proteomics using SILAC.
Functional and quantitative proteomics using SILAC.
Nat Rev Mol Cell Biol. 2006 Dec ;7(12):952-8.

PubMed
Do we have a clinically useful breast cancer risk reduction agent for healthy wo...
Do we have a clinically useful breast cancer risk reduction agent for healthy women?
Nat Clin Pract Oncol. 2006 Dec ;3(12):650-1.

PubMed
Genome-wide natural antisense transcription: coupling its regulation to its diff...
Genome-wide natural antisense transcription: coupling its regulation to its different regulatory mechanisms.
EMBO Rep. 2006 Dec ;7(12):1216-22.

PubMed
Innovative approaches to anti-arrhythmic drug therapy.
Innovative approaches to anti-arrhythmic drug therapy.
Nat Rev Drug Discov. 2006 Dec ;5(12):1034-49.

PubMed
Mining the Wnt pathway for cancer therapeutics.
Mining the Wnt pathway for cancer therapeutics.
Nat Rev Drug Discov. 2006 Dec ;5(12):997-1014.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: