DTRAF1 is required specifically for Mira, but not for Pon, telophase rescue in insc NBs (A–R). Lateral view of stage 10 NBs labeled with anti-Mira (green) or anti-Pon (red). In DTRAF1 mutant NBs, Mira and Pon form basal crescents at metaphase (B, D) and are segregated into future GMCs during telophase (F, H) as in WT NBs (A, C, E, G). In insc mutant NBs, Mira and Pon form extended crescents in metaphase NBs (I, J). During telophase, Mira and Pon are segregated into the future GMCs in the majority of the NBs (N, O). In DTRAF1 insc double-mutant embryos, Mira forms extended crescent or cortical in the majority of dividing NBs during metaphase (K) and by telophase Mira is only partially segregated into the future GMCs, leaving a Mira ‘tail' in the large cell (arrow, P). Whereas Pon is still localized normally in the same dividing NB and segregated exclusively into the future GMC (L, Q). Superimposed images of (K), (L) and (P), (Q) (M, R). Apical is up, DNA is in blue. White dots outline the cell body.