Identification of homologous genes of T. annulata proteins in the genome of Theileria sp. (China)

Ann N Y Acad Sci. 2006 Oct:1081:468-70. doi: 10.1196/annals.1373.067.

Abstract

Homologues to previously described Theileria (T.) annulata genes (T. annulata surface protein [TaSP], putative T. annulata membrane protein [TaD]) were successfully amplified by polymerase chain reaction (PCR) from Theileria sp. (China) merozoite cDNA, with 88% identity to TaD; TcSP partial cDNA, 94% identity to TaSP. Moreover, homologues to a secretory protein of T. annulata (TaSE), with a sequence identity of 99% on the cDNA level (TcSE partial cDNA) and to a potential membrane protein of T. lestoquardi (Clone-5), with a sequence identity of 100% on the genomic level (Tc Clone-5) but lacking an intron at positions 1894-1928 were identified.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Gene Amplification
  • Gene Library
  • Genes, Protozoan*
  • Genome, Protozoan / genetics*
  • Membrane Proteins / genetics
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods
  • Polymerase Chain Reaction / veterinary
  • Protozoan Proteins / genetics*
  • Protozoan Vaccines
  • Sequence Homology, Amino Acid
  • Theileria annulata / genetics*
  • Theileriasis / diagnosis*
  • Theileriasis / parasitology
  • Theileriasis / prevention & control

Substances

  • Membrane Proteins
  • Protozoan Proteins
  • Protozoan Vaccines
  • TaSP protein, Theileria annulata