Department of Biochemistry and Cell Biology, SUNY at Stony Brook, Stony Brook, New York, USA.
The identification of novel forms of O-linked glycosylation on epidermal growth factor and thrombospondin type 1 repeats, and their emerging functional significance, require the development of new methods for their analysis. This chapter describes detailed methods to analyze both the structure and the site of modification of O-fucose and O-glucose glycans on proteins. These methods use both traditional biochemical methods of carbohydrate composition analysis and electrospray ionization-mass spectrometry of glycopeptides.