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Methods Enzymol. 2006;417:93-111.

Methods for analysis of O-linked modifications on epidermal growth factor-like and thrombospondin type 1 repeats.

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  • 1Department of Biochemistry and Cell Biology, SUNY at Stony Brook, Stony Brook, New York, USA.

Abstract

The identification of novel forms of O-linked glycosylation on epidermal growth factor and thrombospondin type 1 repeats, and their emerging functional significance, require the development of new methods for their analysis. This chapter describes detailed methods to analyze both the structure and the site of modification of O-fucose and O-glucose glycans on proteins. These methods use both traditional biochemical methods of carbohydrate composition analysis and electrospray ionization-mass spectrometry of glycopeptides.

PMID:
17132500
[PubMed - indexed for MEDLINE]
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