Abstract
Enhancing the nutritional value of food crops is a means of improving human nutrition and health. We report here the positional cloning of Gpc-B1, a wheat quantitative trait locus associated with increased grain protein, zinc, and iron content. The ancestral wild wheat allele encodes a NAC transcription factor (NAM-B1) that accelerates senescence and increases nutrient remobilization from leaves to developing grains, whereas modern wheat varieties carry a nonfunctional NAM-B1 allele. Reduction in RNA levels of the multiple NAM homologs by RNA interference delayed senescence by more than 3 weeks and reduced wheat grain protein, zinc, and iron content by more than 30%.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Cloning, Molecular
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Frameshift Mutation
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Genes, Plant*
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Iron / metabolism*
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Molecular Sequence Data
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Plant Leaves / chemistry
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Plant Proteins / metabolism*
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Plants, Genetically Modified
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Protein Structure, Tertiary
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Quantitative Trait Loci
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RNA Interference
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RNA, Plant / genetics
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RNA, Plant / metabolism
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Transcription Factors / chemistry
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Transcription Factors / genetics*
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Transcription Factors / physiology
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Triticum / chemistry
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Triticum / genetics*
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Triticum / metabolism*
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Triticum / physiology
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Zinc / metabolism*
Substances
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Plant Proteins
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RNA, Plant
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Transcription Factors
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Iron
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Zinc
Associated data
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GENBANK/DQ869672
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GENBANK/DQ869673
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GENBANK/DQ869674
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GENBANK/DQ869675
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GENBANK/DQ869676
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GENBANK/DQ869677
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GENBANK/DQ869678
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GENBANK/DQ869679
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GENBANK/DQ871219